Adenosine Diphosphate Requirement in the Creatine Phosphate-Induced Contraction of the Glycerinated Rabbit Psoas
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منابع مشابه
Adenosine Diphosphate Requirement in the Creatine Phosphate-Induced Contraction of the Glycerinated Rabbit Psoas
Glycerinated rabbit psoas fibers were tested for their ability to contract under the influence of creatine phosphate and creatine kinase in the absence of free nucleotide. Tension development by the fibers was observed upon addition of creatine phosphate to the medium containing creatine kinase purified to the first lyophilization stage. However, when the enzyme was washed free of nucleotides b...
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Force (F) and stiffness (K) were measured in glycerinated psoas fibers at various calcium levels with 0, 10, 20, and 30 mM orthophosphate (Pi) added to the bathing solutions. The concentrations of bathing solution constituents were as follows: 110 mM potassium, 40 mM sodium, 4 mM MgATP, 10 mM total EGTA, and variable amounts of MOPS (pH buffer). The pH was 7.0, the ionic strength was 200 mM, an...
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With suitable submitochondrial particles from the heart, the rate of the inorganic phosphate e HOH exchange during substrate oxidation in the absence of ADP is less than l/1000 of the rate in the presence of ADP. The concentration dependence of the exchange on ADP shows simple saturation behavior with an apparent K, of 0.32 mM. For net oxidative phosphorylation under similar conditions, but wit...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1962
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.46.2.215